MonoRabᵀᴹ 一款耐高盐Flag纯化柱结合量高Anti-DYKDDDDK Affinity Resin，载量高达1.5mg/ml

产品中心

产品货号：L00766-5ml

产品品牌：南京金斯瑞生物

规格：5m

价格：¥8980

收藏：

收起
展示

概述

Description

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

Key Features

Product content	50% settled resin in TBS with 50% glycerol and 0.02% sodium azide
Matrix	4% cross-linked agarose
Average bead size	90 μm
Ligand	MonoRabᵀᴹ monoclonal antibody against DYKDDDDK tag
Binding capacity	Approximately 1.5 mg DYKDDDDK-tagged protein (Size: 50KDa) per ml settled resin
Storage and stability	Store at -20 °C for up to 12 months.
Resin reuse	The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method	Alkaline, peptide competitive or SDS-PAGE loading buffer elution*
Reagents compatibility	Compatible with commonly used reagents at certain concentrations

*When eluting with SDS-PAGE loading buffer, the heavy chain of the MonoRabTM antibody will be denatured and released from the resin.

特性

Storage

Store at -20 °C for up to 12 months.

相关产品

产品编号	名称	价格
RP10586-1	DYKDDDDK Peptide - 1mg	¥300
L00455W	DYKDDDDK Tag Antibody Plate (White, 96-well) - 5plates	¥2290
L00455C	DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5plates	¥2290
L00455B	DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5plates	¥2290
L00432-1	Anti-DYKDDDDK G1 Affinity Resin - 1ml	¥2810
L00432-25	Anti-DYKDDDDK G1 Affinity Resin - 25ml	¥25910
L00432-5	Anti-DYKDDDDK G1 Affinity Resin - 5ml	¥6630
L00432-10	Anti-DYKDDDDK G1 Affinity Resin - 10ml	¥11930
L00425	Anti-DYKDDDDK IP Resin - 1ml	¥1990
A01811-100	THE™ DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse - 100μg	¥1200
A01810-100	THE™ DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse - 100μg	¥1200
A01809-100	THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100μg	¥1200
A01632	THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100μg	¥1200
A01429-100	THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100μg	¥1200
A01428-100	THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100μg	¥1200
A00187-100	THE™ DYKDDDDK Tag Antibody, mAb, Mouse - 100μg	¥1000
A00170-40	DYKDDDDK-tag Antibody, pAb, Rabbit - 40μg	¥990

产品编号: L00766
	规格	价格
	1 ml	¥3740
	5 ml	¥8980
	25 ml	待询价

暂无资料下载

发文献申领奖金啦！点击这里了解更多——
2023-06-04
LncRNA Tmem235调控BMSC缺氧性凋亡促进激素性股骨头坏死修复
2023-05-23

参考文献1：www.baidu.com

产品编号	中文名称	产品规格	价格