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畅销款耐还原剂Ni填料组氨酸标签纯化High Affinity Ni-Charged Resin FF, Ni-NTA-6%琼脂糖,Binding capacity >40 mg of histidine-tagged protein /ml settled resin
产品货号:L00666-200ml
产品品牌:南京金斯瑞生物
规格:200ml
价格:¥9900
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Description GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins (see Figure 1) [1]. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.
Key Features Characteristics of High Affinity Ni-Charged Resin FF
Matrix spherical 6% highly cross-linked agarose
Average particle size 90 µm (45-165 µm)
Dynamic Binding capacity1 ≥50 mg of histidine-tagged protein /mL settled resin
Storage solution  20% ethanol

1 Dynamic binding capacity conditions:
Sample: 5 mg/mL (histidine)6-tagged pure proteins (Mr 43 000) in binding buffer (capacity at 10%  Breakthrough)
Column volume: 1 mL 
Retention time: 3min
Flow rate: 0.333 mL/min
Binding buffer: 20 mM sodium phosphate, 500 mM NaCl, 10 mM imidazole, pH 7.4
Elution buffer: 20 mM sodium phosphate, 500 mM NaCl, 250 mM imidazole, pH 7.4 
Note: Dynamic binding capacity is protein-dependent.
Reagents Compatible with High Affinity Ni-Charged Resin FF
Reducing agents Denaturants Detergents Salts Others
1 mM DTT 6 M Gua·HCl 2% Triton X-100 4 M MgCl2 50% glycerol
20 mM β-ME        8 M Urea   2% Tween 20 5 mM CaCl2 20% ethanol
5mM TCEP-HCl —— 50% glycerin 2 M NaCl 1 mM EDTA
10 mM reduced glutathione —— —— 100mM Na2SO4 60 mM citrate

† Tested for 1 week at 40°C. 
 The strong chelator EDTA has been used successfully in some cases at 1 mM. Generally, chelating agents should be used with caution (and only in the sample, not in buffers). Any metal ion stripping may be counteracted by addition of a small excess of MgCl2 before centrifugation/filtration of the sample. Note that stripping effects may vary with applied sample volume.
Product Information
Cat. No. Product Name Volume supplied Resin content
L00666-5 High Affinity Ni-Charged Resin FF 5 ml 10 ml 50% slurry
L00666-25 High Affinity Ni-Charged Resin FF 25 ml 50 ml 50% slurry
L00666-100 High Affinity Ni-Charged Resin FF 100 ml 200 ml 50% slurry

 

特性 

Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.

 

例证 

  • High Affinity Ni-Charged Resin FF
  • 产品编号: L00666
     

    规格

    价格

    5 ml ¥403
    25 ml ¥1646
    100 ml ¥5519
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